A Method to Analyze Urinary Extracellular Vesicles
نویسندگان
چکیده
منابع مشابه
Aquaporins in Urinary Extracellular Vesicles (Exosomes)
Since the successful characterization of urinary extracellular vesicles (uEVs) by Knepper's group in 2004, these vesicles have been a focus of intense basic and translational research worldwide, with the aim of developing novel biomarkers and therapeutics for renal disease. Along with these studies, there is growing evidence that aquaporins (AQPs), water channel proteins, in uEVs have the poten...
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Although nanosized urinary extracellular vesicles (uEVs) are increasingly used for biomarker discovery, their isolation currently relies on time-consuming techniques hindering high-throughput application. To navigate this problem, we designed an immunoassay to isolate, quantify, and normalize uEV proteins. The uEV immunoassay consists of a biotinylated CD9 antibody to isolate uEVs, an antibody ...
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Background & Aims: Extracellular vesicles are double phospholipid layer vesicles releasing from several cells. These particles contain bio-molecules including mRNA, miRNA, DNA, proteins, and lipids which play a pivotal role in the cell to cell communication. Once delivered, they participate to change target cell function, fate, morphology, differentiation, and growth. The present study is a re...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2020
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.20r001